Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives
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Authors: Sadeghpour Heravi F, Zakrzewski M, Vickery K, G Armstrong D, Hu H
Publication: Journal of clinical medicine
Year: 2019
Link: https://www.ncbi.nlm.nih.gov/pubmed/31717640
ABSTRACT
Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosis. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganisms. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA- and RNA-based techniques for bacterial identification can improve therapeutic approaches. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches.
- Listing ID: 6696
- Author/s: Sadeghpour Heravi F, Zakrzewski M, Vickery K, G Armstrong D, Hu H
- Publication: Journal of clinical medicine
- Year: 2019
- Volume: 8
- Issue: 11
- Article Keywords: 16S rRNA sequencing; culture; culturomics; diabetic foot infections; diabetic foot ulcers; metagenomics; metatranscriptomics; microbiology; microbiota